Primary colonization and breakdown of igneous rocks by endemic, succulent elephant trees (Pachycormus discolor) of the deserts in Baja California, Mexico

Trees growing in rocks without soil are uncommon. In two arid regions in Baja California, Mexico, field surveys found large numbers of rock-colonizing elephant trees (Pachycormus discolor (Benth.) Coville ex Standl. (Mexican name: copalquin) growing in igneous rocks (granite and basalt) as primary colonizers without the benefit of soil or with a very small amount of soil generated by their own growth. Many adult trees broke large granite boulders and were capable of wedging, growing in, and colonizing rocks and cliffs made of ancient lava flows. This is the first record of a tree species, apart from the previously recorded cacti, capable of primary colonization of rocks and rock rubble in hot deserts.     

Spectral characterization of two bioaccumulated methoxylated polybrominated diphenyl ethers

Two methoxylated polybrominated diphenyl ethers (MeO-PBDEs) were isolated from a True's beaked whale (Mesoplodon mirus) and identified by NMR (1H, 1H-1H and 1H-13C) and high resolution mass spectrometry as 2-(2',4'-dibromophenoxy)-3,5-dibromoanisole (6-MeO-BDE47) and 2-(2',4'-dibromophenoxy)-4,6-dibromoanisole (2'-MeO-BDE68). Previously the structures of these bioaccumulated compounds have been determined by comparison of their mass spectra and gas chromatographic (GC) retention times with those of authentic standards. While this method is accepted and generally successful, NMR of the isolated compounds allows us to definitively identify the congeners. Our characterizations are consistent with those made for MeO-PBDEs in other organisms, identified by chromatographic methods.     

Enzymatic degradation of anthracene, dibenzothiophene and pyrene by manganese peroxidase in media containing acetone

The high hydrophobicity of polycyclic aromatic hydrocarbons (PAHs) greatly hamper their degradation in liquid media. The use of an organic solvent can assist the degradative action of ligninolytic enzymes from white rot fungi. The enzymatic action of the enzyme manganese peroxidase (MnP) in media containing a miscible organic solvent, acetone (36% v/v), was evaluated as a feasible system for the in vitro degradation of three PAHs: anthracene, dibenzothiophene and pyrene. These compounds were degraded to a large extent after a short period of time (7, 24 and 24h, respectively), at conditions maximizing the MnP-oxidative system. The initial amount of enzyme present in the reaction medium was determinant for the kinetics of the process. The order of degradability, in terms of degradation rates was as follows: anthracene>dibenzothiophene>pyrene. The intermediate compounds were determined using gas chromatography-mass spectrometry and the degradation mechanisms were proposed. Anthracene was degraded to phthalic acid. A ring cleavage product of the oxidation of dibenzothiophene, 4-methoxybenzoic acid, was also observed.     

Anguilla anguilla L. oxidative stress biomarkers: an in situ study of freshwater wetland ecosystem (Pateira de Fermentelos, Portugal)

Pateira de Fermentelos (PF) is a natural freshwater wetland in the central region of Portugal. In the last decade, the introduction of agricultural chemicals, heavy metals, domestic wastes, as well as eutrophication and incorrect utility of resources resulted in an increased water pollution. The present research work was carried out to check the various oxidative stress biomarker responses in European eel (Anguilla anguilla L.) gill, kidney and liver due to this complex water pollution. Eels were caged and plunged at five different PF sites (A-E) for 48h. A reference site (R) was also selected at the river spring where no industrial contamination should be detected. Lipid peroxidation (LPO), catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST) and reduced glutathione (GSH) were the oxidative stress biomarkers studied. In gill, site A exposure induced a significant GST activity increase and site B exposure induced CAT activity increase when compared to R. Site C exposure showed a significant CAT and GPX activity increase. Data concerning site D exposure were not determined due to cage disappearance. Site E exposure displayed a significant CAT and GST activity increase. In kidney, site A exposure induced a significant CAT and GPX decrease as well as a GST increase. Site B exposure showed a significant decrease in GPX activity and GSH content. However, site C exposure demonstrated a significant increase in CAT and a decrease in GPX. Site E exposure showed a significant decrease in GPX and increase in GST. In liver, site A exposure showed a significant GST activity decrease as well as GSH content increase. Site B exposure showed a significant CAT, GST and LPO decrease. Site C exposure showed only GST activity decrease, while site E exposure induced a significant increase in GPX. These investigation findings provide a rational use of oxidative stress biomarkers in freshwater ecosystem pollution biomonitoring using caged fish, and the first attempt reported in Portugal as a study of this particular watercourse under the previous conditions. The presence of pollutants in the PF water was denunciated even without a clear relation to the main pollution source distance. The organ specificity was evident for each parameter but without a clear pattern.     

Soil remediation time to achieve clean-up goals I: Influence of soil water content

The current models are not simple enough to allow a quick estimation of the remediation time. This work reports the development of an easy and relatively rapid procedure for the forecasting of the remediation time using vapour extraction. Sandy soils contaminated with cyclohexane and prepared with different water contents were studied. The remediation times estimated through the mathematical fitting of experimental results were compared with those of real soils. The main objectives were: (i) to predict, through a simple mathematical fitting, the remediation time of soils with water contents different from those used in the experiments; (ii) to analyse the influence of soil water content on the: (ii(1)) remediation time; (ii(2)) remediation efficiency; and (ii(3)) distribution of contaminants in the different phases present into the soil matrix after the remediation process. For sandy soils with negligible contents of clay and natural organic matter, artificially contaminated with cyclohexane before vapour extraction, it was concluded that (i) if the soil water content belonged to the range considered in the experiments with the prepared soils, then the remediation time of real soils of similar characteristics could be successfully predicted, with relative differences not higher than 10%, through a simple mathematical fitting of experimental results; (ii) increasing soil water content from 0% to 6% had the following consequences: (ii(1)) increased remediation time (1.8-4.9h, respectively); (ii(2)) decreased remediation efficiency (99-97%, respectively); and (ii(3)) decreased the amount of contaminant adsorbed onto the soil and in the non-aqueous liquid phase, thus increasing the amount of contaminant in the aqueous and gaseous phases.     

Mineralisation of 2,6-dichlorobenzamide (BAM) in dichlobenil-exposed soils and isolation of a BAM-mineralising Aminobacter sp

Mineralisation of the groundwater contaminant 2,6-dichlorobenzamide (BAM), a metabolite from the herbicide 2,6-dichlorobenzonitrile (dichlobenil), was studied in soil samples obtained from 39 locations previously exposed to dichlobenil. Rapid BAM mineralisation was detected in samples from six locations with 5.2-64.6% of the added BAM mineralised within 48-50 days. From one location rapid BAM mineralisation was observed in soil samples down to a depth of 2 m below the surface. One location with fast BAM mineralisation showed significant dichlobenil degradation activity with 25.5% of the added dichlobenil being mineralised within 50 days. By inoculating soil showing the fastest mineralisation of BAM into a mineral medium with BAM as the only carbon and nitrogen source an enrichment culture was established. Community analysis based on extracted DNA revealed a change of the bacterial community but without any clear indication of key members within the BAM-mineralising culture. Parallel cultivation resulted for the first time in the isolation of a BAM-mineralising bacterium, identified as an Aminobacter sp.     

The Brazilian National Intercomparison Program (PNI/IRD/CNEN): evaluation of 15 years of data

The Instituto de Radioprote??o e Dosimetria has been coordinating the National Intercomparison Program (PNI) for 15 years, from 1991 to 2005. The objective is to evaluate the analytical performance of the laboratories in low-level activity concentration radionuclide assays in environmental samples. This work presents an evaluation of PNI data from 42 intercomparison runs, which distributed to 22 Brazilian laboratories 2511 samples evaluating 5768 radionuclide assays involving 32 radionuclides in the period from 1991 to 2004. The laboratory performance was evaluated using the Normalized Standard Deviation used by the U.S. EPA. For comparison aims, the Normalized Deviation, used by BIPM was also applied. Laboratory performance were grouped and evaluated in three periods of 5 years each. The first period shows, an average value of good performance of 71.2%, the second shows an average of 78.6% and the last an average of 82.3%. Performance for each kind of radionuclide is also presented.     

Downstream and Coastal Impacts of Damming and Water Abstraction in Africa

Anthropogenic factors associated with damming and water abstraction, and the resultant environmental pressures, are reviewed in six African river catchments using records and forecasts of climatic, demographic, and land-use change. Changes in the states of the flow regime through catchment drainage systems to the coastal sea are considered in conjunction with climate change and other human-induced pressures. The impacts of these changes on downstream and coastal environments and their communities are described in past, present, and future perspectives. Linkages between the issues and the pressures of damming and water abstraction are appraised and scientific, policy, and management responses proposed aimed at remedying existing and perceived future negative impacts. The study proposes that there is a need to integrate catchment and coastal management to account for the whole water flow regime together with its human dimensions. Management priorities relating to the operation of existing damming and abstraction schemes and planning of future schemes include the following: consideration of ways in which water discharges could be adjusted to provide improvements in downstream and coastal environmental and socioeconomic conditions; addressing the problem of sediment trapping impacting on the sustainability of dam reservoirs; and assessment of downstream and coastal impacts of future schemes in the light of climate change forecasts.     

Ecophysiological Competence of <Emphasis Type="Italic">Populus alba</Emphasis> L., <Emphasis Type="Italic">Fraxinus angustifolia</Emphasis> Vahl., and <Emphasis Type="Italic">Crataegus monogyna</Emphasis> Jacq. Used in Plantations for the Recovery of Riparian Vegetation

In many semi-arid environments of Mediterranean ecosystems, white poplar (Populus alba L.) is the dominant riparian tree and has been used to recover degraded areas, together with other native species, such as ash (Fraxinus angustifolia Vahl.) and hawthorn (Crataegus monogyna Jacq.). We addressed three main objectives: (1) to gain an improved understanding of some specific relationships between environmental parameters and leaf-level physiological factors in these riparian forest species, (2) to compare the leaf-level physiology of these riparian species to each other, and (3) to compare leaf-level responses within native riparian plots to adjacent restoration plots, in order to evaluate the competence of the plants used for the recovery of those degraded areas. We found significant differences in physiological performance between mature and young white poplars in the natural stand and among planted species. The net assimilation and transpiration rates, diameter, and height of white poplar plants were superior to those of ash and hawthorn. Ash and hawthorn showed higher water use efficiency than white poplar. White poplar also showed higher levels of stomatal conductance, behaving as a fast-growing, water-consuming species with a more active gas exchange and ecophysiological competence than the other species used for restoration purposes. In the restoration zones, the planted white poplars had higher rates of net assimilation and water use efficiency than the mature trees in the natural stand. We propose the use of white poplar for the rapid restoration of riparian vegetation in semi-arid Mediterranean environments. Ash and hawthorn can also play a role as accompanying species for the purpose of biodiversity.     

Optimization of "Serpa" cheese whey nanofiltration for effluent minimization and by-products recovery

Second cheese whey (SCW) is a by-product of cheese and curd cheese production that is usually not recovered and therefore substantially contributes to the negative environmental impact of the cheese manufacture plants. Membrane technology, namely nanofiltration (NF), is used in this work for the recovery of SCW organic nutrients, resulting from "Serpa" cheese and curd production. The SCW is processed by NF to recover a rich lactose fraction in the concentrate and a process water with a high salt content in the permeate. The permeation experiments were carried out in a plate & frame NF unit, where two NF membranes (NFT50 and HR-95-PP) were characterized and tested. The NF permeation experiments were performed accordingly with two different operation modes: total recirculation and concentration. In order to select the best membrane and operating pressure for the SCW fractionation, total recirculation experiments were carried out. The NF modeling was also performed, in terms of permeate fluxes and rejection coefficients using the resistances-in-series model and the solution-diffusion model, respectively. After the membrane selection, the concentration experiments showed that the selected membrane (NFT50) at 3.0MPa allows a water recovery of approximately 80%, concentrating the SCW nutrients approximately 5 times. Therefore, the NF operation can successfully reduce the wastewater organic load and simultaneously contributes to the valorization of the cheese and curd cheese manufacture by-products.